Biophysics
At Proteros we have built an extensive biophysics platform that enables you to generate the target-ligand interaction results and insight that you need and allow you to REACH RIGHT FASTER.
Our Biophysics platforms enable:
- Biophysical assays for target-ligand interaction analysis
- Library screening (incl fragments)
- Quantification of binding affinity and/or kinetics: Kd, kon, koff, kinact, ∆S, ∆H, ∆G
- Orthogonal hit verification
- Site of ligand binding
BIOPHYSICS TECHNOLOGIES, DELIVERABLES, APPLICATIONS, AND THROUGHPUT
Surface Plasmon Resonance (SPR)
Technology: 8K + instrument (Biacore)
Deliverable: Ligand binding, Kd, kon, koff, residence time
Applications: Primary screen (incl. fragments), hit profiling, lead optimization, investigation of ternary complex formation
Throughput: Screen: up to 20,000 ligands/week, Kd: up to 100 ligands/week
Isothermal Calorimetry Titration (ITC)
Technology: Malvern Microcal PEAQ ITC®
Deliverables: Kd, ∆S , ∆ H , N
Applications: Hit profiling, orthogonal screen, lead optimization, candidate selection, verification of allosteric binders
Throughput: Kd: up to 10 ligands/week
nanoDSF
Technology: PrometheusTM (NanoTemper technologies) (label free)
Deliverable: TmApplications: Primary Screen (incl. fragments), orthogonal screen, allosteric screen, testing of protein stability, construct evaluation, investigation of protein domain structure
Throughput: Screen: up to 1,500 ligands/week
TSA
Technology: Thermoshift assays (based on dyes)
Deliverable: Tm
Applications: Primary Screen (incl. fragments), orthogonal screen, allosteric screen, testing of protein stability, construct evaluation, investigation of protein domain structure
Throughput: Screen: up to 20,000 ligands/week
MicroScaleThermophoresis (MST)
Technology: Monolith NT115picoTm (NanoTemper technologies)
Deliverable: KdApplications: Primary screen (incl. fragments), hit profiling, lead optimizationThroughput:
Screen: up to 1,500 ligands/week, Kd: up to 50 ligands/week
Profiling technology
Proteros Statistics
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years unlocking technically demanding targets
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Pharma and biotech partners
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Protein structures elucidated
Why use the Proteros Biophysics services
Assay Development and screening
Expertise to match assay and Biophysics strategy to required insights
Access to fit-for-purpose screening libraries
High-Quality Screening libraries
Agility and flexibility of execution & communication
Dedicated, expert project management
Contact Us
Contact us to find out more about the Proteros biophysics services.
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