PROTEIN SCIENCES
- Construct design
- Expression systems: E.coli, insect, mammalian, yeast
- Purification and rigorous analytics-driven Quality Control
Reach right faster to obtain Cryo-EM structures
What if there was an integrated platform that combined world class protein science with cryo-EM infrastructure?
There is! We call it the Proteros Integrated Cryo-EM Workbench.
- Dedicated team for protein construct design, expression and purification with rigorous quality control for cryo-EM
- EM feasibility analysis (including negative staining EM)
- Cryo-EM condition optimization and structure determination
- Special target expertise for various target classes
Our Cryo-EM publications
EM FEASIBILITY ANALYSIS
- Purity, Homogeneity and Stability testing
- Negative Staining EM
- Ligand-Protein interaction analysis
STRUCTURE DETERMINATION
- Cryo-EM condition optimization
- Low-Resolution cryo-EM and/or
- High-Resolution cryo-EM
Protein for cryo-EM
- Construct design
- Gene synthesis
- Expression
- Purification
- Analytics-driven Quality Control
- Product and data delivery
EM feasibility Analysis
- Negative staining EM
- 2D classification
- EM feasibility (to prove homogeneity and complex stability)
- Data driven decision on "how-to-proceed"
- Optimization of cryo-EM grid freezing conditions and Cryo-EM 2D classification
First Structure
- Collection of 12 h data sets (Glacios)
- Data processing and structure refinement
- Low-resolution cryo-EM structure (3D envelope, < 10 Å)
- X-ray structures can be fitted into density
- Visualization of protein/complex architecture
- Visualization of antibody-antigen interaction
High-resolution cryo-EM
- Collection of 24 - 72 h datasets (Glacios & Krios)
- Data processing and structure refinement
- High-resolution cryo-EM structure (visible side-chains, < 4 Å)
- Visualization of ligand-binding mode
- Validation of binding mode
- Enables structure-based drug design